Validation of a multiplex reverse transcriptase PCR ELISA for the detection of 19 respiratory tract pathogens
نویسندگان
چکیده
منابع مشابه
Multiplexed reverse transcriptase PCR assay for identification of viral respiratory pathogens at the point of care.
We have developed a nucleic acid-based assay that is rapid, sensitive, and specific and can be used for the simultaneous detection of five common human respiratory pathogens, including influenza virus A, influenza virus B, parainfluenza virus types 1 and 3, respiratory syncytial virus (RSV), and adenovirus groups B, C, and E. Typically, diagnosis on an unextracted clinical sample can be provide...
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Avian influenza virus (AIV) infection is a major cause of bird or human mortality and morbidity, therefore the rapid identification of the virus is of important clinical and epidemiological implication. Methods: A multiplex Reverse Transcriptase PCR (RT-PCR) was optimized for the detection of influenza A virus and the H5 and H9 subtypes. The influenza type A specific primers were directed to t...
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OBJECTIVES Lower respiratory tract infection is one of the most important causes of morbidity and mortality. However establishing a microbial diagnosis for patients with lower respiratory tract infection is still challenging and is often achieved in only half of cases by conventional methods. This study was designed to compare the fast responsive PCR method with the culture method in lower resp...
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ژورنال
عنوان ژورنال: Infection
سال: 2012
ISSN: 0300-8126,1439-0973
DOI: 10.1007/s15010-012-0298-6